Northern Blot problem

Karen.Straube-West Karen.Straube-West
Mon Aug 21 12:40:10 EST 1995


Hi Bionetters,

We are suddenly having difficulty with our Northern blots using total RNA.
The appropriate bands are present, but they are smeary. Even the rRNA on
the gel looks smeary. It isn't bad RNA because it looks fine on a
non-denaturing gel.

We've checked, and the pH of our formaldehyde is about 3.2. Our protocol
says it should be >4.0. Question: Could this be the problem? If so, can we
raise the pH of our formaldehyde or should we buy new stuff? I'd appreciate
any advice.

Thanks,

Dr. Karen Straube-West
SUNY Health Science Center
750 East Adams Street
Syracuse, NY 13210






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