SDS-PAGE HELP

Giorgio Spagnol spagnol at galactica.it
Mon Aug 21 05:32:51 EST 1995


Dear fellow researcher,
I don't know if everybody agree on this, but I always tought the sample
(loading) buffer in one dimensional SDS-PAGE should have a pH of 6.8. But
what about the solution in which your sample proteins are diluted? Last
time I diluted them in the same solution in which Bio-Rad MW are (300 mM
NaCl, 0.1 M DTT, no buffer) then I added anti-proteases ( Leupeptin,
Pepstatin, Aprotinin, EDTA) and they did not enter the gel. Does anybody
knows whay?
In short, what should be the ionic strenght and the pH of your's protein
solution?
Any help woul be greatly appreciated.
Giiorgio



More information about the Methods mailing list