DNA concentration

"Marianne Leverone ", BIO leverone at CHUMA.CAS.USF.EDU
Mon Aug 21 09:21:57 EST 1995


Giorgio:  yes;  add an equal amount (vol) of butanol to your DNA soln.; 
vortex; centrifuge; take off top layer (butanol layer).  Repeat this 
until your DNA soln. has decreased to the desired volume.  Problems with 
this protocol are:  shearing of DNA during vortexing and remaining 
butanol in DNA soln.  The latter can be removed by either ppt. the DNA or 
drawing off some of the DNA soln. when the butanol is aspirated. 

On 17 Aug 1995, Giorgio Spagnol wrote:

> Dear fellow scientists,
> do you know any good protocol to concentrate DNA, apart from
> precipitating it?
> Any suggestion would be greatly welcome.
> Thanks in advance.
> Giorgio
> 
> 



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