DNA concentration

Richard Rohan rrohan at world.std.com
Mon Aug 21 21:26:13 EST 1995


Giorgio Spagnol <spagnol at galactica.it> wrote:

>Dear fellow scientists,
>do you know any good protocol to concentrate DNA, apart from
>precipitating it?
>Any suggestion would be greatly welcome.
>Thanks in advance.
>Giorgio

1. Lyophilization 

2. Repeated butanol extraction - After the second or third extraction, the
butanol will begin to remove aqueous.  Caution: Butanol partitions on the top!

The first two will concentrate salts as well as DNA.  Which leads to ...

3. Spin dialysis - such as centricon or microcon (Amicon) columns.


---
Rich Rohan, PhD (rrohan at world.std.com)
Independent Molecular Biology Consultant to the Internet
Moderator REPRENDO at world.std.com - Reproductive Endocrinology List




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