Restriction digestion of PCR fragments

Adrian Molenaar Molenaara at Ruakura.CRI.NZ
Tue Aug 22 00:12:41 EST 1995


Try two phenol extractions of your PCR product before restricting it. 
Taq (and sufficient nucleotides) sticks to to the nucleotides even
through ethanol precipitation in sufficinent quantitites to nodify your
newly cut ends, thus preventing succesful ligation

Adrian Molenaar

Molenaara at Agresearch.cri.nz



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