Separating cut from uncut vector

Ira M. Lubin mediml at
Tue Aug 22 22:01:22 EST 1995

I'm looking for suggestions on how to effectively separate a 12kb  vector 
fragment from uncut vector.  0.7%-1.0% agarose gel
electrophoresis is not sufficient. Enough uncut vector remains to 
adversely effect subsequent ligation/transformation.



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