Help Priming with Low Tm primers

Rob Fialho rfialho at moose.uvm.edu
Tue Aug 22 14:30:56 EST 1995


I designed these 18 mer primers to amplify a 700 or so bp fragment but 
they dont seem to work under any condidtions, I get multiple bands 
anywhere between 48-50 C. Their Tm's are 50 and 54 C.  I have lowered the 
magnesium concentration to 1 mM and still got faint multiple bands.  How 
can I get around this?  I'd aprreciate any suggestions!  Thanks!

Rob




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