Genomic DNA isolation

Dr Saleem Mohammed saleem at rfhsm.ac.uk
Tue Aug 22 01:30:08 EST 1995


Hi,

Seem to be having a few problems with shearing when I am isolating genomic DNA.
The protocol is the traditional RNAse/proteinase K/phenol extraction method.  
All the tips for pipettes are cut to ensure minimisation of sgearing effects, 
but still the problem persists.

Can anyone suggest any modifications
Dr Saleem Mohammed,
Dept of Biochemistry & Molecular Biology,
Royal Free Hospital School of Medicine.
Rowland Hill Street,
London. NW2 2PF.



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