green fluorescent protein (GFP)
gki2 at mailserv.uni-giessen.de
Tue Aug 22 09:22:18 EST 1995
Is there anyone out there who has had success in expressing GFP in
If so, what kind of expression vector / transfection method /
cells have you used and what is your transfection efficiency?
I am interested in the cellular distribution pattern of my protein of
interest which is synthetized, secreted and bound by endothelial cells.
've extensively tried ito localize this protein by immunofluorescence but
the signal is (in spite of biot./stav. amplification) not very strong - so I'd
like to overexpress the protein in endothelial cells.
Now I'm not sure if it's really an advantage to use GFP-fusions because
there were a lot of, let's say contradictory reports around. GFP would
be very nice not only for detection in living cells but as a tool for
Any comments / suggestions will be greatly appreciated.
THANX FOR READING THIS - GREETINGS
Ivica Cerina, PhD e-mail: gki2 at kerckhoff.med.uni-giessen.de
Haemostasis Research Unit
MPI f. physiol. u. klin. Forschung
61231 Bad Nauheim
(I know this sounds very optimistic but are there any reports on stably
transfected cells producing GFP fusion proteins?)
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