Dr I.M. Clark
imc1001 at cus.cam.ac.uk
Tue Aug 22 09:38:44 EST 1995
Nigel Kenward (mbxnjk at vax.nott.ac.uk) wrote:
: Hi There,
: Does anyone have a simple method for detecting CAT in a positive
: transfection. I don't have a toxicity curve for the cell line I'm using,
: and I don't want to use readioactive Acetyl CoA.
: Surely there's a nice enzymatic assay out there isn't there ?
: Thanx in anticipation,
: Nigel Kenward TEL:+44 (0)115 9249924 ex. 44789
: Dept. of Biochemistry FAX:+44 (0)115 9422225
: Queens Medical Centre Email:mbxnjk at vax.nott.ac.uk
: Clifton Boulevard
: U.K. "Back off man, I'm a scientist!" -- Bill Murray.
Boehringer-Mannheim (no relation) sell a CAT ELISA which works well.
Ian M. Clark
Rheumatology Research Unit,
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