CAT detection

[Dr I.M. Clark]

Nigel Kenward (mbxnjk at vax.nott.ac.uk) wrote:
: Hi There,
: Does anyone have a simple method for detecting CAT in a positive 
: transfection. I don't have a toxicity curve for the cell line I'm using, 
: and I don't want to use readioactive Acetyl CoA.
: Surely there's a nice enzymatic assay out there isn't there ?
: Thanx in anticipation,
: Nigel

: Nigel Kenward              TEL:+44 (0)115 9249924 ex. 44789
: Dept. of Biochemistry      FAX:+44 (0)115 9422225
: Queens Medical Centre      Email:mbxnjk at vax.nott.ac.uk
: Clifton Boulevard          
: Nottingham						
: U.K.                   "Back off man, I'm a scientist!"  -- Bill Murray.


Boehringer-Mannheim (no relation) sell a CAT ELISA which works well.
Ian
--
Ian M. Clark
Rheumatology Research Unit,
Addenbrooke's Hospital,
Cambridge. UK.