DNA concentration

Stephen R. Lasky, Ph.D. Stephen_Lasky at brown.edu
Tue Aug 22 10:06:05 EST 1995


In article <Pine.SUN.3.91.950821101436.13910A-100000 at chuma>,
leverone at CHUMA.CAS.USF.EDU ("Marianne Leverone ", BIO) wrote:

> Giorgio:  yes;  add an equal amount (vol) of butanol to your DNA soln.; 
> vortex; centrifuge; take off top layer (butanol layer).  Repeat this 
> until your DNA soln. has decreased to the desired volume.  Problems with 
> this protocol are:  shearing of DNA during vortexing and remaining 
> butanol in DNA soln.  The latter can be removed by either ppt. the DNA or 
> drawing off some of the DNA soln. when the butanol is aspirated. 
> 
> On 17 Aug 1995, Giorgio Spagnol wrote:
> 
> > Dear fellow scientists,
> > do you know any good protocol to concentrate DNA, apart from
> > precipitating it?
> > Any suggestion would be greatly welcome.
> > Thanks in advance.
> > Giorgio
> > 
> > 

You can do this with dry diethyl ether as well and it forms a nicer
interface than butanol.  But if you are going to vortex the DNA, why not
just ppt it?  

SRLasky

********************************************************************
Stephen R. Lasky Ph.D.  Brown University/Roger Williams Medical Center
e-mail:  Stephen_Lasky at brown.edu  LandLine: 401-456-6572  FAX: 401-456-6569
^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
America may be unique in being a country which has leapt from barbarism to decadence without touching civilization.
-- John O'Hara
********************************************************************



More information about the Methods mailing list