REP-PCR primer purity

L. S. del Rosario ldelrosario at
Mon Aug 21 16:14:32 EST 1995

Our lab is starting up rep-PCR for DNA fingerprinting of various types of pathogenic 
bacteria. We are in the process of purchasing primers and wanted to find out 
suggestions on recommendation for primer set-up. Should our primers be 
fully deprotected as well as desalted? Other recommedations for primer set up would 
also be appreciated.  

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