CAT detection

Nigel Kenward mbxnjk at vax.nott.ac.uk
Tue Aug 22 02:50:42 EST 1995


Hi There,
Does anyone have a simple method for detecting CAT in a positive 
transfection. I don't have a toxicity curve for the cell line I'm using, 
and I don't want to use readioactive Acetyl CoA.
Surely there's a nice enzymatic assay out there isn't there ?
Thanx in anticipation,
Nigel

Nigel Kenward              TEL:+44 (0)115 9249924 ex. 44789
Dept. of Biochemistry      FAX:+44 (0)115 9422225
Queens Medical Centre      Email:mbxnjk at vax.nott.ac.uk
Clifton Boulevard          
Nottingham						
U.K.                   "Back off man, I'm a scientist!"  -- Bill Murray.





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