Need GTPase for positive control

[Cornelius Krasel]

Dima Klenchin (klenchin at macc.wisc.edu) wrote:
> >In article <newitt-1008951734290001 at 128.231.113.35>, 
> newitt at nih.gov says...
> >>I am doing some GTPase assays and would like to obtain an 
> enzyme with
> >>intrinsic GTPase activity to use as a positive control.

> John, retinal transducin, I think, would be the best thing.
> If you need it as a control, there is no need to purify the 
> protein. Bovine rods contain which are very easy to 
> purify contain HUGE amounts of it. 

AFAIK transducin is different from other G proteins in that it has
no GTPase activity when unstimulated. So you have to illuminate
the rods to get transducin activity (BTW a nice control).

--Cornelius.

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