Need GTPase for positive control
krasel at wpxx02.toxi.uni-wuerzburg.de
Tue Aug 22 03:14:33 EST 1995
Dima Klenchin (klenchin at macc.wisc.edu) wrote:
> >In article <newitt-1008951734290001 at 18.104.22.168>,
> newitt at nih.gov says...
> >>I am doing some GTPase assays and would like to obtain an
> enzyme with
> >>intrinsic GTPase activity to use as a positive control.
> John, retinal transducin, I think, would be the best thing.
> If you need it as a control, there is no need to purify the
> protein. Bovine rods contain which are very easy to
> purify contain HUGE amounts of it.
AFAIK transducin is different from other G proteins in that it has
no GTPase activity when unstimulated. So you have to illuminate
the rods to get transducin activity (BTW a nice control).
/* Cornelius Krasel, U Wuerzburg, Dept. of Pharmacology, Versbacher Str. 9 */
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