Best primers for ABI sequencing of Bluescript
RM Brownlie
rmb10 at unix.york.ac.uk
Wed Aug 23 10:17:11 EST 1995
I have inserts cloned into the EcoRI/XhoI site of the Bluescript II SK
vector and would like to know which are the best primers for Dye
Terminator cycle sequencing using an ABI automated sequencer. Perkin and
Elmer recommend the M13 forward and reverse primers but the reverse
primer is a considerable distance from the EcoRI site. Has anyone had
much success using the T7 or T3 standard primers or modifications of and
did they modify any of the standard conditions? I would also like to know
whether it is worth doing single strand rescue to obtain maximum read
lengths?
Any information regarding achieving optimum results would be gratefully
received.
Robert Brownlie
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