SYBR Green I

Suzuki Kazuhiko kazu at bio.hokudai.ac.jp
Wed Aug 23 03:04:08 EST 1995


Hi, netter
Have someone experienced DNA extraction from 
non-denatured polyacrylamide gel electrophoresis stained by SYBR Green I?
I hear SYBR Green I is very sensitive and have ability to detect 
very small quantity of nucleotides.
So, I suspect the extracted product is proper for ligation, 
sequencing (fluorescent, in paticular).
Is the purity and quantity of the products O.K?
Is there no need for the contamination of SYBR Green I ?


Kazuhiko SUZUKI
Graduate School of Science
Division of Biological Sciences
Hokkaido Univ.




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