Genomic DNA isolation

Francois Nantel Nantel at titus.u-strasbg.fr
Wed Aug 23 01:59:24 EST 1995


In article <saleem.30.00048BF0 at rfhsm.ac.uk> Dr Saleem Mohammed,
saleem at rfhsm.ac.uk writes:
>Hi,
>
>Seem to be having a few problems with shearing when I am isolating
genomic DNA.
>The protocol is the traditional RNAse/proteinase K/phenol extraction
method.  
>All the tips for pipettes are cut to ensure minimisation of sgearing
effects, 
>but still the problem persists.
>
During the phenol extraction, it is important to keep the rotation at
less than 1 rpm. This was not evident in most of the protocols I read.

Hope this helps

Francois



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