Separating cut from uncut vector
John K. Troyer
jtroyer at umabnet.ab.umd.edu
Wed Aug 23 08:12:56 EST 1995
On 23 Aug 1995, Ira M. Lubin wrote:
> I'm looking for suggestions on how to effectively separate a 12kb vector
> fragment from uncut vector. 0.7%-1.0% agarose gel
> electrophoresis is not sufficient. Enough uncut vector remains to
> adversely effect subsequent ligation/transformation.
Non-denaturining acrylamide gels (5-10%) give MUCH better separation than
agarose gels. The vector can be separated and eluted from the gel in much the
same way as you do with agarose.
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