Separating cut from uncut vector

John K. Troyer jtroyer at umabnet.ab.umd.edu
Wed Aug 23 08:12:56 EST 1995


On 23 Aug 1995, Ira M. Lubin wrote:

> I'm looking for suggestions on how to effectively separate a 12kb  vector 
> fragment from uncut vector.  0.7%-1.0% agarose gel
> electrophoresis is not sufficient. Enough uncut vector remains to 
> adversely effect subsequent ligation/transformation.
> 
> Thanks,
> 
> Ira


Non-denaturining acrylamide gels (5-10%) give MUCH better separation than 
agarose gels.  The vector can be separated and eluted from the gel in much the 
same way as you do with agarose. 


John 



More information about the Methods mailing list