Separating cut from uncut vector

[Andy Kravetz]

John K. Troyer (jtroyer at umabnet.ab.umd.edu) wrote:

: Non-denaturining acrylamide gels (5-10%) give MUCH better separation than 
: agarose gels.  The vector can be separated and eluted from the gel in much the 
: same way as you do with agarose. 

John, I am having that very problem. I am trying to do a ligation and 
well, there is a still a lot of uncut vector. If you do use PAGE, then 
don't you have to do the crush and soak method of DNA purification. Right 
ow, I just run it out on the gel and cut the band out, leaving it in the 
LM agarose. 

Help, I am at my wit's end trying to do this damn ligation. Thanks,

andy

--

------------------------------------
"I love the wild power of language and and the purity of the madness that
governs it and makes it music" 
                                        -HST, _Generation of Swine_
Andy Kravetz
Freelance Journalist
St. Louis, Missouri
akravetz at walden.mo.net
http://walden.mo.net/~akravetz/