What to use to elute DNA binding protein from DNA ?
S Chen Lab
jzhang at biovax.rutgers.edu
Wed Aug 23 17:27:10 EST 1995
The mobility of a labeled probe is retarded when incubated with a
nuclear extract. I'd like to enrich for the presumed DNA binding
protein by using tethered DNA to capture that component of the nuclear
extract. My question: is there a general buffer to elute (unknown) DNA
binding proteins from their binding site -- into solution for further
analysis. After looking at a couple of random papers at hand, I am
thinking of 100mM NaOAC 4.2, or simply very high salt like 4M NaCl or
KCl. After the protein is in solution, I'd concentrate and exchange
the buffer for subsequent SDS-PAGE. All I'd like to see for now is the
size of the protein.
Thanks for any buffer suggestions.
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