photographing GFP

Ted M. tedm at
Wed Aug 23 22:44:33 EST 1995

In article <1995Aug18.090058.19263 at>, futers at

> Hi,
> Has anyone any experience of photographing cells that are expressing
> GFP (green fluorescent protein?  What is the best light source 
> for excitation and is it necessary to use any filters?
> Many thanks
>        Simon Futers  (futers at

A bandpass filter of ~488nm over a very bright incandescent source like a
slide projector works well for  excitation while a ~520nm bandpass filter
allows viewing the green glow on a totally black background, provided you
have a std. GFP species. Such filters could probably be fitted to a camera
and they cost $200-$300 from such places as Edmund Scientific (or Fisher

PS excitation could also be from a single line Argon laser (also 488nm) if
photobleaching is avoided.


Ted Michelini
Institute of Molecular Biology
University of Oregon
tedm at

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