Help with Growth Media Precipitation
rtate at bmb-fs1.biochem.okstate.edu/
Thu Aug 24 10:18:25 EST 1995
I use a synthetic media to grow bacteria in order to define the
vitamins and cofactors present. I use charcoal treated Casein, various
vitamins, and salts and KPO4 to buffer at pH 7. I have made this and
grown many many batches of bacteria with no problems until recently. I
am now having a problem with a precipitate forming when I adjust the pH.
It appears to be CaPO4. It also precitates if casein and KPO4 are the
only ingredients present or if I adjust the pH to near 7 before I add
the KPO4. I have tried another source of water with the same results.
Any suggestions will be greatly appreciated.
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