Mutagenesis

Joanne D. Andreadis jandread at umabnet.ab.umd.edu
Thu Aug 24 11:16:36 EST 1995


I've been trying rather unsuccessfully to introduce an amber codon in the 
middle of my gene of interest in a standard pET vector. I've tried using 
several methods including the Chang et al. double pcr protocol and 
commercial kits such as Stratagene's ex-cite mutagenesis to no avail.  I'm 
changing two ntds and this seems like it should  be very simple. Has anyone 
else tried a nice mutagenesis protocol with decent results. Thanks. 



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