Restriction digestion of PCR fragments

Robert Horton horton at
Thu Aug 24 11:03:51 EST 1995

Adrian Molenaar (Molenaara at Ruakura.CRI.NZ) wrote:
: Try two phenol extractions of your PCR product before restricting it. 
: Taq (and sufficient nucleotides) sticks to to the nucleotides even
: through ethanol precipitation in sufficinent quantitites to nodify your
: newly cut ends, thus preventing succesful ligation

Do you have a reference for this claim? (Not that I don't believe it, I
just can't find a reference...). Thanks.

Robert M. Horton (PhD!) /\ "Crash programs fail because of the theory that
U of M Dermatology Dept || with nine women pregnant you get a baby a month"
Box 98 UMHC, 4-154 PWB /||\ -W. von Braun.   Disclaimer: "Bob who?"
Minneapolis, MN 55455   ^^   horton at       (612)625-8941

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