Quantitative PCR

[Frank Chen]

No matter how you do it and how good your controls are, your results from 
quantitative PCR will be questionable.  My suggestion is not to use this 
approach if you can.  Please be aware that this is only my suggestion.

Frank Chen


On 24 Aug 1995, sabah hussain wrote:

> Dear netters. My basic problem is that the mRNA species that I am looking for (nitric oxide synthase mRNA) is expressed in a small abundance in the tissues that I am 
> interested in. My northern blot analysis was disappointing. I am therefore relying on PCR to quantitate the message. I am looking for the best protocol for quantitative 
> PCR. My second question is whether using GAPDH or active primers along with the specific primers is adequate to control for differences in tempelate RNA in different 
> samples. I appreciate any help in this matter.
> 
> 
>