kappei at basic2.kpu-m.ac.jp
Fri Aug 25 20:53:21 EST 1995
I'm considering the design of antigene oligodeoxynucleotide (ODN)
to control stress gene expression. I intend to synthesize 13-mer ODN
consisted of thymine and 5-methyl-cytosin and 5'-linked acrydine,
which is expected to form triplex on stress 70 promoter.
I hope someone to tell me whether or not
1)phosphorothioate modification of the ODN is needed?
2)a single mismatch at 3' terminus reduce the stability of expected
3)there are some other strategies (or modification) of ODN to improve it
in the ability to shutt off the gene expression?
Other recent info on antigene ODN would be appreciated.
Thanks in advance
Kappei Kobayashi (kappei at basic2.kpu-m.ac.jp)
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