Which cDNA library kit?
amarion at moose.uvm.edu
Fri Aug 25 18:03:29 EST 1995
I am in the process of choosing a kit for synthesis of a cDNA library.
Which do you prefer? Stratagene Lambda Zap kit or Promega Riboclone kit using
the Pormege Lambda GEM-4 vector?
I have worked with libraries made in Lambda Zap and I like being able
to excise the Bluescript plasmid from the phage. The Riboclone system seems
to offer the same opportunity by releasing the pGEM-1 vector from the phage.
My reading of current molecular genetic literature indicates many (most?)
people are using Lambda Zap. There must be a reason for this. But... the
Promega system would be cheaper (a serious consideration at this time).
If there is a system better than Lambda Zap or Riboclone I'd like to
hear about that, too.
Fort Wayne, Indiana
marion at smtplink.ipfw.indiana.edu
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