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proteinase K solutions

Rafael Maldonado rafael at howard.genetics.utah.edu
Fri Aug 25 13:48:42 EST 1995

On 24 Aug 1995, Howard T. Petrie wrote:

> We use proteinase K for DNA/RNA purification, etc.  We make it up at
> 20mg/ml in water and store it frozen, as all of the prominent methods
> texts recommend.  

So your proteinase K precipitated...

It happened to me all the time. B-M proteinase K precipitate when it is 
cold at 20 mg/ml concentration. We tried 5 mg/ml and the precipitation 
stopped. It is a pain get dissolved the proteinase K again, but I never 
detected a loss of activity when precipitation is present.

I realized that other proteinases from different companies don't have got 
that problem. Currently, I am using Gibco-BRL at 20 mg/ml without any 
precipitation problem. 

No relation with any of those companies, economical, sentimental, 
emotional nor musical.


Rafael Maldonado                             |  La cita ha sido
room 6160 Eccles Institute of Human Genetics |  
Department of Human Genetics		     |  retirada por respeto
University of Utah			     |
Salt Lake City, Utah 84112. USA.	     |  a la propiedad 
Rafael.Maldonado at genetics.utah.edu           |
Rafael at howard.genetics.utah.edu	             |  intelectual.
Tel: 801-581-4429			     |
Fax: 801-585-3910			     |

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