On 24 Aug 1995, Howard T. Petrie wrote:
> We use proteinase K for DNA/RNA purification, etc. We make it up at
> 20mg/ml in water and store it frozen, as all of the prominent methods
> texts recommend.
So your proteinase K precipitated...
It happened to me all the time. B-M proteinase K precipitate when it is
cold at 20 mg/ml concentration. We tried 5 mg/ml and the precipitation
stopped. It is a pain get dissolved the proteinase K again, but I never
detected a loss of activity when precipitation is present.
I realized that other proteinases from different companies don't have got
that problem. Currently, I am using Gibco-BRL at 20 mg/ml without any
No relation with any of those companies, economical, sentimental,
emotional nor musical.
Rafael Maldonado | La cita ha sido
room 6160 Eccles Institute of Human Genetics |
Department of Human Genetics | retirada por respeto
University of Utah |
Salt Lake City, Utah 84112. USA. | a la propiedad
Rafael.Maldonado at genetics.utah.edu |
Rafael at howard.genetics.utah.edu | intelectual.
Tel: 801-581-4429 |
Fax: 801-585-3910 |