DNA "refusing" to be run in agarose gel
Edward E. Diehl
mcdon at mc.duke.edu
Fri Aug 25 15:25:31 EST 1995
You might also want to add some salt (NaCl) to your samples prior to
loading. I have observed the same problem using Wizard-prep minipreps and
was able to stop the "suck out" problem by adding one µl of 1M NaCl to the
sample. I also think that ethanol is a major problem, be sure to dry your
DNA completely prior to restriction enzyme digestion.
> On Fri, 25 Aug 1995, Ketil Thorstensen wrote:
> > Honored contributors to the molbio.methds-reagnts!
> > I have a peculiar problem with persuading some of my DNA samples to run i=
> > agarose gels.
> > When loading DNA samples on agarose gels I have on several occasions obse=
> > a strange (to me) phenomenon: after application of a sample into a well a=
> > streak of the sample may follow the pipette tip upon withdrawal. When the=
> > sample in this way comes in contact with the buffer surface, the sample v=
> > rapidly spreads out to cover the entire surface. During this process most=
> > the sample in the well is "sucked" out.
> > There is no recognizable pattern as to what sample this may happen with. =
> > is, however, frustrating when precious samples are lost (the last one was=
> > 1.9 kb DNA probe) :=ED-(
> > Could anyone enlighten me as to what I am observing, and how to prevent=
> > this from happening again?
> > Thank you very much,
> > Ketil Thorstensen, Ph.D.
> > Dept. Clinical Chemistry
> > University Hospital
> > Trondheim, Norway
> > =20
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