TE saturation of chloroform/isoamyl alcohol?
drm21 at mole.bio.cam.ac.uk
Fri Aug 25 19:29:23 EST 1995
In article <41iij9$svu at news.duke.edu>, Margon Vandongen
<vando005 at mc.duke.edu> wrote:
>Definitely true!! May I suggest though you buy this already
>made (Gibco sells it), it is a mess to have to do it yourself.
>No attachments etc.
Is this really true? I've never TE saturated my chloroform (I don't
bother with the isoamyl alcohol) and haven't noticed any problems...
Now phenol's another matter entirely! I definitely agree that its better
to get this pre-equilibrated (let alone un-redistilled, ugh). True, it is
much more expensive per liter, but then I get through far far less than a
bottle a year, so the total expense is small...
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