Quantitation of in vitro syn RNA

Chuck Hauser chauser at acpub.duke.edu
Fri Aug 25 16:09:30 EST 1995


I need to quantitate pmoles of T7 synthesized 32P-labelled RNA, as well as
unlabelled invitro synthesized RNA. What are the preferred methods, do you
gel purify the RNA, or NH4Ac ppt from the synthesis rxn? thanks



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