InsituPCR to polytenes

pdxrmb at vaxb.nott.ac.uk pdxrmb at vaxb.nott.ac.uk
Fri Aug 25 10:36:25 EST 1995


Has anyone got advice on using In Situ PCR to detect sequences on the
polytene chromosomes of D. melanogaster. We are cuurently usinf Vectors Bio
tin ISH system ,and have tried a few pilot experiments with biotin-utp
spiked PCR mixes and the standard detection protocol, but without success.
The chromosomes are good for ISH, but  we havent got any specific signals.
Any help would be much appreciated- thanks in advance- Richard M. Badge



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