proteinase K solutions
Howard T. Petrie
h-petrie at ski.mskcc.org
Thu Aug 24 11:04:12 EST 1995
We use proteinase K for DNA/RNA purification, etc. We make it up at
20mg/ml in water and store it frozen, as all of the prominent methods
texts recommend. In the past (we have historically purchased it from
B-M), there has always been a precipitate in this solution. We recently
purchased some from Stratagene, and there is no precipitate at the same
concentration. What are other peoples' experiences with proteinase K in
solution? Are precipitates common? I would also be interested in hearing
specifically from people who use Stratagene's proteinase K. If you don't
want to respond in public, my e-mail address is
h-petrie at pop.ski.mskcc.org. Thanks.
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