Help on HPLC and Prostaglandin E2

Sailesh Surapureddi SaiSu at MCB.LiU.SE
Fri Aug 25 03:49:38 EST 1995


>To: methods-and-reagents at net.bio.net
>From: baldoni.1 at postbox.acs.ohio-state.edu (sjgouldrulesmiggy)
>Subject: Help on HPLC and Prostaglandin E2
>Date: Thu, 24 Aug 1995 16:04:52 -0400
>
>Netters,
>   Does anyone know the wavelength of PGE2 ?????
>
>regards,
>miggy

Hi,
	Yes, the wavelengths I used to work for PGs are between 196-205 depending on 
the column type, i,e manufactures prescription, the choosing exact wave length 
in between these two nos was determined for each expt basing on the UV spectral 
analysis of the samples. But if you are only looking for PGE2 then I would stick 
to 200nm.
Hope you have good separations.
Regards
Sailesh.



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