DNA mismatch repair
mcdon at mc.duke.edu
Fri Aug 25 11:31:35 EST 1995
In article <9508251553.AA08099 at intnet.upj.com>, rvdeshp0 at INTNET.UPJ.COM wrote:
> If there is a nucleotide mismatch in a double stranded DNA, is this
> mismatch corrected in the sense or the anti-sense strand ? Or is
> there an equal probability of repair on each strand ? Any help will
> be appreciated. Thanks.
> Rajendra V. Deshpande, Ph.D.
> Molecular Biology Research
> The Upjohn Company
> Kalamazoo, Michigan
> e-mail : rvdeshp0 at intnet.upj.com
Got it right the second time. Assuming you are transforming into E.coli
to repair the mismatch (and I can't think of any other way), it's 50-50
where the repair will be. You can sequence to find the correct mutation,
or design it so a resriction site is created.
Dr. Benjamin Lieberman
liebe003 at mc.duke.edu
More information about the Methods