effect of 3' UTR on recombinant expression
Sergey V. Orlov
Serge at serge.stud.pu.ru
Sat Aug 26 17:57:24 EST 1995
Niels Groot wrote:
> I'm trying to express a recombinant protein but till now expression
> levels are rather low: +/- 200 ng /ml.
> This low expresion levels are expression system independent: sofar I
> tried COScells / BHK cells (pcDNAI under CMV promoter control) and the
> Baculovirus sytem. I don't think this is the result of instability of
> the recombinant protein itself or due to degradation by proteases.
> The cDNA clone I use lacks most part of the '3 UTR sequence. Is it
> possible that this lack of the '3 UTR causes instability of the
> recombinant RNA and hence low expression levels?
Indeed, the 3' UTR can cause instability of the mRNA. Recently, sequence
mediating this degradation have been determined. It is nonamer UUAUUUAUU.
For details, see reference: A.M. Zubiaga et al. Mol. Cell. Biol. 1995
Vol.15, N.4, pp.2219-2230
With best wishes, Serge Orlov
E-mail: serge at serge.stud.pu.ru
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