Can you run an RNA TBE-agarose gel?

Margon Vandongen vando005 at mc.duke.edu
Sat Aug 26 10:42:26 EST 1995


John,

We routinely run RNA gels to see if we have in vitro transcripts.
We use 1% agarose gels in TAE. We presoak the boxes in 2% H2O2 for
about 10 minutes. Ethidiumbromide is included in gel and running
buffer (just make the gel from runningbuffer). We do have a special
stock of buffer that is DEPC treated and autoclaved.
Of course you cannot estimate sizes but just to see if there is RNA
present this is a quick way to do it. 

Good Luck, Margon

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