Can you run an RNA TBE-agarose gel?
vando005 at mc.duke.edu
Sat Aug 26 10:42:26 EST 1995
We routinely run RNA gels to see if we have in vitro transcripts.
We use 1% agarose gels in TAE. We presoak the boxes in 2% H2O2 for
about 10 minutes. Ethidiumbromide is included in gel and running
buffer (just make the gel from runningbuffer). We do have a special
stock of buffer that is DEPC treated and autoclaved.
Of course you cannot estimate sizes but just to see if there is RNA
present this is a quick way to do it.
Good Luck, Margon
-------------- next part --------------
A non-text attachment was scrubbed...
Name: not available
Size: 0 bytes
Desc: not available
Url : http://iubio.bio.indiana.edu/bionet/mm/methods/attachments/19950826/772cb443/attachment.txt
More information about the Methods