growing bacteria in microtite
carsonj at ozemail.com.au
Sun Aug 27 07:07:51 EST 1995
nater at gargamel.rug.ac.be (Nancy Terryn) wrote:
>has anybody out there tried to grow bacteria in microtiterplates in
>a small volume like 200 microliter? I just had a funny result with that.
>All the bacteria in the middle of the plate grew well but the onces on the outer rows did not grow? How can this be explained? I did not shake the plates, i just put them at 37.
I grow a variety of bacteria in identification media in 96 well microtitre
plates in 100 micro litre volumes with no 'edge' effects. I wonder whether
evaporation may be a problem? I use sterile flat bottomed Nunclon plates for
tissue culture. After inoculation I seal the plate with adhesive film (ICN make
a tape on a roll that is the width of a tray. Incidentally, these trays are
used for minimum inhibitory concentration antibiotic assays without a problem.
More information about the Methods