?? ROBOCYCLER ??
100537.271 at CompuServe.COM
Mon Aug 28 11:13:22 EST 1995
Regarding the Robocycler:
I write from Techne(UK) to pass on the experience we have with
testing the Robolcycler.
We find the idea of moving the sample tubes from block to block
does not infact remove the ramping time as the time is required
for the thermal transfer of energy through to the sample.
At Techne we find the fastest crossover of heat to sample is
approx 3°C/sec,(thin wall tube 0.2ml and 0.5ml), this does
increase of course as the tube temperature increases and becomes
slightly more conductive.
A number of the faster Thermal cyclers on the market now ramp at
speeds of 3°C/sec, including the Techne GeneE and the new,
(available September), XXX cycler. Because the ramping speeds are
equial to the thermal transfer rate the Robocycler offers no
advantage on this point.
Another finding was that the sample actually experienced a drop
in sample temperature whilst being transfered between blocks,
thus increasing the possibility of non-specific reactions
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