DNA "refusing" to be run in agarose gel
rpa.groot.oncol at med.vu.nl
Mon Aug 28 08:39:25 EST 1995
In article <ECS9508251125D at utoronto.ca>, r.lombardi at utoronto.ca (Anyname) says:
>> I have a peculiar problem with persuading some of my DNA samples
>to run in
>> agarose gels.
>> When loading DNA samples on agarose gels I have on several
>> a strange (to me) phenomenon: after application of a sample into
>a well a
>> streak of the sample may follow the pipette tip upon withdrawal.
>> sample in this way comes in contact with the buffer surface, the
>> rapidly spreads out to cover the entire surface. During this
>process most of
>> the sample in the well is "sucked" out.
>> There is no recognizable pattern as to what sample this may
>happen with. It
>> is, however, frustrating when precious samples are lost (the
>last one was a
>> 1.9 kb DNA probe) :í-(
>> Could anyone enlighten me as to what I am observing, and how to
>> this from happening again?
>> Thank you very much,
>> Ketil Thorstensen, Ph.D.
>> Dept. Clinical Chemistry
>> University Hospital
>> Trondheim, Norway
>This, or something like this, sometimes happens if the sample
>being loaded has just been ethanol precipitated and the ethanol
>was not completely evaporated. That little bit of ethanol is
>enough to make your resuspended DNA sample float.
>Dept. of Microbiology
>U. of Toronto
>r.lombardi at utoronto.ca
It also happens with, mineral oil overlayed, PCR products.
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