DNA "refusing" to be run in agarose gel

Bixing Huang huangbx at DEAKIN.EDU.AU
Mon Aug 28 02:09:57 EST 1995


It happened to me sometimes. But I change to TBE. This annoying problem
disappear!!!

Bixing Huang

>I think there are 2 possible causes-- one is residual ethanol in the
>sample (if it was precipitated and the pellet not dried). The other is if
>there is a huge amount of genomic DNA in the sample that is not digested.=
=20
>Could either be the case?
> Adam Greene
>
>On Fri, 25 Aug 1995, Ketil Thorstensen wrote:
>
>> Honored contributors to the molbio.methds-reagnts!
>>=20
>> I have a peculiar problem with persuading some of my DNA samples to run=
 in=20
>> agarose gels.
>>=20
>> When loading DNA samples on agarose gels I have on several occasions
observed=20
>> a strange (to me) phenomenon: after application of a sample into a well a=
=20
>> streak of the sample may follow the pipette tip upon withdrawal. When the=
=20
>> sample in this way comes in contact with the buffer surface, the sample=
 very=20
>> rapidly spreads out to cover the entire surface. During this process most=
 of=20
>> the sample in the well is "sucked" out.
>> There is no recognizable pattern as to what sample this may happen with.=
 It=20
>> is, however, frustrating when precious samples are lost (the last one was=
 a=20
>> 1.9 kb DNA probe)  :=ED-(
>> Could anyone enlighten me as to what I am observing, and how to prevent=
=20
>> this from happening again?
>>=20
>> Thank you very much,
>>=20
>> Ketil Thorstensen, Ph.D.
>> Dept. Clinical Chemistry
>> University Hospital
>> Trondheim, Norway
>> =20
>>=20
>>=20
>
>
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