Why? In vivo exsicion didn't work.
Mari L. Shinohara
Mari.L.Shinohara at dartmouth.edu
Mon Aug 28 15:50:47 EST 1995
I recently had a problem on in vivo excision of DNA insert in lambda
ZAP phage. I did not get any E. coli colonies then. Though it has
been solved anyhow fortunately, I'd like to know what was the
--- I used the XL1-blue strain grown on LB+Tet plate and followed the
protocol supplied by Strategene. I tried it 5 or 6 times, but no
colonies appeared. I was desperate and got XL1-blue from my friend,
which was also grown on LB+Tet just I did. Then... It worked. I did
the prep exactly in the same way. Well... May be I vortexed tubes
after adding lambda and helper phages together with bacterial cells,
when I was using my E. coli.
Is it possible that my bacterial cells had some problem even though I
had used Tet selection? Or vortexing made my experiment ruined?
Your help will be greatly appreciated!
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