growing bacteria in microtite
PRASHERD at DELPHI.COM
PRASHERD at news.delphi.com
Mon Aug 28 15:41:47 EST 1995
nater at gargamel.rug.ac.be (Nancy Terryn) writes:
When bacterial cultures are grown in microtiter plates incubated at 37 C,
a LOT of the liquid evaporates from those wells near the outside edges.
To minimize the evaporation, incubate the plates inside a sealed
container (Tupperware is good). Inside the container lay a moistened
paper towel such that the atmosphere is saturated with water.
prasherd at delphi.com
>has anybody out there tried to grow bacteria in microtiterplates in
>a small volume like 200 microliter? I just had a funny result with that.
>All the bacteria in the middle of the plate grew well but the onces on the outer rows did not grow? How can this be explained? I did not shake the plates, i just put them at 37.
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