direct PCR amplifiction using library

dinakar bhattramakki ddh at unlinfo.unl.edu
Mon Aug 28 14:36:05 EST 1995


I am looking for a detailed protocol for dirct PCR using cDNA library 
as the DNA source. I am using denatured (boiling for 5 min) 1ul phage stock
(approx. 100,000 pfu) in PCR reaction. Unfortunately I am not getting 
any amplification product. Only one of the primers is specific for the
gene of my interest, another is T7 or T3 primer.

Thanks in advance
-Dinakar 

--
- Dinakar



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