thomas.kreuzer at kfunigraz.ac.at
Thu Aug 24 20:43:36 EST 1995
On Thu. 24 Aug Frank Chen wrote as a reply to a question concerning
" No matter how you do it and how good your controls are , your results from
quantitative PCR will be questionable."
Since I am trying to put up a quantitative RT-PCR - method myself (and being
quite new to the method of PCR in common) I would be interested in the major
reservations against the principle of quantitative PCR and especially
quantitative RT-PCR. What exactly makes it´s results questionable and why
can´t these problems be avoided ?
It would be a great help if you could also give me some advice about
literature dealing with these problems.
Thanks in advance,
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