Protein Expression

Darren Fast fast at
Mon Aug 28 14:10:41 EST 1995

In article
<Pine.A32.3.91.950825200434.41130A-100000 at>,
berns001 at wrote:

> The protein I want to study will more than likely immediately bind the 
> DNA of the organism used to express it, thus killing said organism.  
> Any suggestions for a way around this?

can you tag a signal sequence onto the protein so that it enters the
secretory pathway and is secreted?  This should work if you are using a
eukaryotic system.


Darren Fast
fast at
Dept of Biochemistry
University of Wisconsin-Madison

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