Protein Expression

[Darren Fast]

In article
<Pine.A32.3.91.950825200434.41130A-100000 at san_marcos.csusm.edu>,
berns001 at coyote.csusm.edu wrote:

> The protein I want to study will more than likely immediately bind the 
> DNA of the organism used to express it, thus killing said organism.  
> 
> Any suggestions for a way around this?

can you tag a signal sequence onto the protein so that it enters the
secretory pathway and is secreted?  This should work if you are using a
eukaryotic system.

Darren

-- 
Darren Fast
fast at biochem.wisc.edu
Dept of Biochemistry
University of Wisconsin-Madison
(608)-262-4705