johnstoj at acs2.acs.ucalgary.ca
Tue Aug 29 11:54:40 EST 1995
brad schonhoft (bschono at magnus.acs.ohio-state.edu) wrote:
: Whatis the best way to store ug quantities of in vitro transcribed RNA -
: it's cold, non-labelled and about 500 bases. Thanks
I think a lot depends on how quickly you are going to use it. We
store it in DEPC water at -80. We keep the number of
thaw/freezes to a minimum and always keep it on ice when it is
out of the freezer.
Hint - if you want to add RNase inhibitors, remember to keep the
DTT level high enough.
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