rna storage

Jillian Johnston johnstoj at acs2.acs.ucalgary.ca
Tue Aug 29 11:54:40 EST 1995


brad schonhoft (bschono at magnus.acs.ohio-state.edu) wrote:
: Whatis the best way to store ug quantities of in vitro transcribed RNA - 
: it's cold, non-labelled and about 500 bases.  Thanks

: Brad
I think a lot depends on how quickly you are going to use it.  We
store it in DEPC water at -80.  We keep the number of
thaw/freezes to a minimum and always keep it on ice when it is
out of the freezer.
Hint - if you want to add RNase inhibitors, remember to keep the
DTT level high enough.  




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