Floureporter to detect LacZ

john sinclair js at mole.bio.cam.ac.uk
Tue Aug 29 12:06:37 EST 1995


We have been using the Molecular Probes Floureporter kit to\
cells transiently transfceted with LacZ  reporter constructs 
with some success.
Does anyone know (i) if it is possible to load ehanol fixed cells 
with the FDG reagent successfully or (ii) if it is possible to fix
the converted fluorescein in the cells, after loading the FDG into
unfixed cells, by ethanol fixation.
Hope someone can help.
Thanks 
John S.



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