John K. Troyer
jtroyer at umabnet.ab.umd.edu
Tue Aug 29 09:45:04 EST 1995
On 28 Aug 1995, Alison Weaver wrote:
> I am having problems with the transfer of a 150KDa protein which
> has a transmembrane domain from SDS PAGE to Hybond C membrane.
> Does anyone have any suggestions how transfer may be enhanced?
Omitting the methanol from the transblot buffer may help the transfer of
higher mol. wt. proteins (and especially glycoproteins) > 100 kDa.
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