Western Blot

John K. Troyer jtroyer at umabnet.ab.umd.edu
Tue Aug 29 09:45:04 EST 1995

On 28 Aug 1995, Alison Weaver wrote:

> I am having problems with the transfer of a 150KDa protein which 
> has a transmembrane domain from SDS PAGE to Hybond C membrane. 
> Does anyone have any suggestions how transfer may be enhanced? 

Omitting the methanol from the transblot buffer may help the transfer of 
higher mol. wt. proteins (and especially glycoproteins) > 100 kDa.


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