TdT vs. KinaseSCDOWN

u2528082 at ucsvc.ucs.unimelb.edu.au u2528082 at ucsvc.ucs.unimelb.edu.au
Sun Aug 27 23:18:25 EST 1995




In article <414rlk$aua at sifon.cc.mcgill.ca>, Constantin Polychronakos <mc97 at musica.McGill.CA> writes:
>>   bickle at ubaclu.unibas.ch (Tom Bickle) writes:
>>  In article <40q8oa$cka at synapse.bms.com>, Steven Goldberg
>>  <goldberg at bms.com> wrote:
>>  
>>  >I have been using TdT for a few years now, and as the other responders
have mentioned, it is more convenient-ie. you don't need to order gamma
isotope, and the specific activity is high.
Using 20-30ul of P32-dATP gives only 0.5-1 label/molecule if labelling say
10pmol, ie 50-60ng of oligo. Thus no need to worry about change in specificity.
Good labelling
John
U2528082 at hermes.unimelb.edu.au

> 



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